Why are these populations different? Flow help!
If these cells are all live, what would cause the difference in FSC/SSC between my WT (left) and mutant (right) mice? This is consistent for the 60 mice I have done, the WTs always have a more compact lymphocyte population, whereas I have increased signal intensity on SSC. Is this due to activation/immune response?
For context, these are splenocyes. There is a 36% effector/activation proportion in the WT and 80% in the mutants.
If these cells are all live, what would cause the difference in FSC/SSC between my WT (left) and mutant (right) mice? This is consistent for the 60 mice I have done, the WTs always have a more compact lymphocyte population, whereas I have increased signal intensity on SSC. Is this due to activation/immune response?
For context, these are splenocyes. There is a 36% effector/activation proportion in the WT and 80% in the mutants.